Analysis Note

Positive ControlSK-BR-3 cells

Application

Flow Cytometry (5 µg/ml, see application references)r>Frozen Sections (5 µg/ml)r>Immunoblotting (see application references)r>Immunofluorescence (5 µg/ml, see application references)r>Immunoprecipitation (1 µg/sample, see application references)r>Neutralization Studies (not recommended, see comments and application references)

General description

Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells.

Anti-c-ErbB2/c-Neu (Ab-5), mouse monoclonal, clone TA-, recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells. It is validated for FC, WB, IF, IP, and IHC on frozen sections.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing spleonocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the c-ErbB2/c-neu protein.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Recognizes an extracellular epitope and does not cross react with human EGF-receptor. It will not inhibit in vivo protein tyrosine kinase activity. Antibody should be titrated for optimal results in individual systems.

DiFiore, P.P., et al. 1987. Science237, 178.Slamon, D.J., et al. 1987. Science235, 177.Varley, J.M., et al. 1987. Oncogene1, 423.Yamamoto, T., et al. 1986. Nature319, 230.Blick, M., et al. 1984. Blood64, 1234.Schwab, M., et al. 1984 Cold Spring Harbor Laboratory2, 215.Bargmann, C.I., et al. 1968. Nature319, 226.

Packaging

100 µg in Plastic ampoule

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM PBS, 0.2% gelatin, pH 7.5.

Warning

Toxicity: Standard Handling (A)